GeneProof Borrelia burgdorferi PCR Kit

CE IVD

PRODUCT DETAIL

The PCR kit is designed for detection of clinically important Borrelia species from the Borrelia burgdorferi sensu lato group causing Lyme disease and for detection of Borrelia miyamotoi causing tick-borne relapsing fever, by the real-time Polymerase Chain Reaction (PCR) method.

The detection consists in amplification of the gene sequence encoding 16S rRNA and in measurement of fluorescence increase. The B. burgdorferi presence is indicated by the FAM fluorophore fluorescence growth. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR by amplification products. The kit is designed for in vitro diagnostics and provides qualitative detection.

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