GeneProof Factor XIII V34L PCR Kit
CE IVD
PRODUCT DETAIL
The PCR kit is designed to detect polymorphism of the F13A1 gene encoding the subunit A of FXIII factor by the real-time Polymerase Chain Reaction (PCR) method.
The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide guanine/thymine polymorphism in site 103 (G103T). Presence of wild-type allele (G103G) is detected in the FAM fluorescent channel and mutant allele (T103T) in the HEX fluorescent channel. In case of heterozygous genotype (G103T) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.
TECHNICAL SPECIFICATION
| Technology | real-time PCR |
| Type of Analysis | qualitative |
| Target Sequence | G103T Polymorphism of the F13A1 gene (V34L) |
| Analytical Sensitivity | reaches up to 2 ng/µl |
| Diagnostic Specificity | 100 % |
| Diagnostic Sensitivity | 100 % |
| Validated Specimen | whole blood |
| Storage | -20 ± 5 °C |
| Validated Extraction Methods | croBEE NA16 Nucleic Acid Extraction System GeneProof PathogenFree DNA Isolation Kit |
| Instruments | croBEE Real-Time PCR System* AriaMx Real-Time PCR System LightCycler® 2.0 LineGene 9600 Plus |
Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / Q
SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR SystemRequired Detection ChannelsFAM, HEXExternal Quality Assessmentregularly tested by Instand e.V. External Quality Assessment panels - results at www.geneproof.comRegulatory StatusCE IVD for in vitro Diagnostic Use
* Validated Instruments
ORDER INFORMATION
| Product name | Cat. No. | Technology | Package |
| GeneProof Factor XIII V34L PCR Kit | FXIII/050 | real-time PCR | 50 reactions |
| GeneProof Factor XIII V34L PCR Kit | FXIII/100 | real-time PCR | 100 reactions |