GeneProof Factor XIII V34L PCR Kit

GeneProof Factor XIII V34L PCR Kit



thrombotic mutations



The PCR kit is designed to detect polymorphism of the F13A1 gene encoding the subunit A of FXIII factor by the real-time Polymerase Chain Reaction (PCR) method.

The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide guanine/thymine polymorphism in site 103 (G103T). Presence of wild-type allele (G103G) is detected in the FAM fluorescent channel and mutant allele (T103T) in the HEX fluorescent channel. In case of heterozygous genotype (G103T) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.



Technology real-time PCR
Type of Analysis qualitative
Target Sequence G103T Polymorphism of the F13A1 gene (V34L)
Analytical Sensitivity reaches up to 2 ng/µl
Diagnostic Specificity 100 %
Diagnostic Sensitivity 100 %
Validated Specimen whole blood
Storage -20 ± 5 °C
Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
Instruments croBEE Real-Time PCR System*
AriaMx Real-Time PCR System
LightCycler® 2.0
LineGene 9600 Plus

Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / Q
SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR SystemRequired Detection ChannelsFAM, HEXExternal Quality Assessmentregularly tested by Instand e.V. External Quality Assessment panels - results at www.geneproof.comRegulatory StatusCE IVD for in vitro Diagnostic Use

* Validated Instruments


Product name Cat. No. Technology Package
GeneProof Factor XIII V34L PCR Kit FXIII/050 real-time PCR 50 reactions
GeneProof Factor XIII V34L PCR Kit FXIII/100 real-time PCR 100 reactions

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