GeneProof Herpes Simplex Virus 2 (HSV-2) PCR Kit

GeneProof Herpes Simplex Virus 2 (HSV-2) PCR Kit



The PCR kit is designed for detection of Herpes simplex virus type 2 (HSV-2) by the real-time Polymerase Chain Reaction (PCR) method.

The HSV-2 detection consists in amplification of a specific conservative DNA sequence of a single-copy gene encoding the glycoprotein B (gB) and in measurements of fluorescence increase. The HSV-2 presence is indicated by the FAM fluorophore fluorescence growth. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA glycosylase (UDG), eliminating possible contamination of the PCR with amplification products. The kit is designed for in vitro diagnostics and provides qualitative and quantitative detection.


Technology real-time PCR
Type of Analysis qualitative and quantitative
Target Sequence specific conservative DNA sequence of a single-copy encoding the glycoprotein B (gB)


Specificity Herpes simplex virus type 2 (HSV-2), 100 %
Sensitivity (LoD) reaches up to 197,215 cp/ml with the probability of 95 % (on AcroMetrixTMHSV-2 Plasma Panel using manual extraction GeneProof PathogenFree DNA Isolation Kit)


Linear Range 1010 - 102.5 cp/ml with precision of ± 0.5 log
Reporting Units cp/μl
Metrological Traceability AcroMetrixTM HSV-2 Plasma Panel (AcroMetrix HSV-2)
Extraction/Inhibition Control PCR inhibition control (ISIN version)

PCR inhibition and DNA extraction efficiency control (ISEX version)

Validated Specimen whole blood, plasma, urine, CSF
Storage -20 ± 5 °C
Validated Extraction Method croBEE NA16 Nucleic Acid Extraction System

GeneProof PathogenFree DNA Isolation Kit

Validated Instruments croBEE Real-Time PCR System
Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System
LightCycler® 2.0 / 480
LineGene 9600 / 9600 Plus

Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / 6000 / Q
SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR SystemRequired Detection ChannelsFAM, HEXQuality ControlQuality management system is certified in compliance with the requirements of the standard ISO 13485External Quality Assessmentregularly tested by QCMD and Instand e.V. External Quality Assessment panels for results see www.geneproof.comCertificationCE IVD for in vitro Diagnostics Use



Product name Cat. No. Technology Package
GeneProof Herpes Simplex Virus 2 (HSV-2) PCR Kit HSV2/ISEX/025 real-time PCR 25 reactions
GeneProof Herpes Simplex Virus 2 (HSV-2) PCR Kit HSV2/ISEX/050 real-time PCR 50 reactions
GeneProof Herpes Simplex Virus 2 (HSV-2) PCR Kit HSV2/ISEX/100 real-time PCR 100 reactions

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