GeneProof Herpes Simplex Virus (HSV-1/2) PCR Kit

CE IVD

PRODUCT DETAIL

The PCR kit is designed for detection and differentiation of both types of Herpes simplex viruses (HSV-1 and HSV-2) by the real-time Polymerase Chain Reaction (duplex PCR) method.

The HSV detection and differentiation consists in amplification of a specific conservative DNA sequence of a single-copy gene encoding the glycoprotein B (gB) and in measurement of fluorescence increase. The HSV-1 presence is indicated by the FAM fluorophore fluorescence growth and the HSV-2 presence is indicated by the Cy5 fluorophore fluorescence growth. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil DNA glycosylase (UDG), eliminating possible contamination of the PCR with amplification products. The kit is designed for in vitro diagnostics and provides qualitative and quantitative detection.

TECHNICAL SPECIFICATION 

QuantStudio™ 5 Real-Time PCR System
Rotor-Gene 3000 / Q
SLAN^® Real-Time PCR SystemRequired Detection ChannelsFAM, HEX, Cy5Quality ControlQuality management system is certified in compliance with the requirements of the standard ISO 13485External Quality Assessmentregularly tested by QCMD and Instand e.V. External Quality Assessment panels - results at www.geneproof.comCertificationCE IVD, for in vitro Diagnostics Use

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