GeneProof MTHFR A1298C PCR Kit

GeneProof MTHFR A1298C PCR Kit


thrombotic mutations



The PCR kit is designed to detect A1298C polymorpism in the gene for methylenetetrahydrofolate reductase (MTHFR) by the real-time Polymerase Chain Reaction (PCR).

The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide adenine/cytosine polymorphism in site 1298 (A1298C). Presence of wild-type allele (A1298A) is detected in the FAM fluorescent channel and mutant allele (C1298C) in the HEX fluorescent channel. In case of heterozygous genotype (A1298C) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.


Technology real-time PCR
Type of Analysis qualitative
Target Sequence A1298C mutation in the gene for methylenetetrahydrofolate reductase (MTHFR)
Analytical Sensitivity reaches up to 2 ng/µl
Diagnostic Specificity 100 %
Diagnostic Sensitivity 100 %
Validated Specimen whole blood
Storage -20 ± 5 °C
Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
Validated Instruments croBEE Real-Time PCR System
Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System
LightCycler® 2.0
LineGene 9600 / 9600 Plus

Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / Q
SLAN® Real-Time PCR SystemRequired Detection ChannelsFAM, HEXExternal Quality AssessmentRegularly tested by Instand e.V. External Quality Assessment panels – results at www.geneproof.comCertificationCE IVD for in vitro Diagnostic Use


Product name Cat. No. Technology Package
GeneProof MTHFR A1298C PCR Kit M1298/050 real-time PCR 50 reactions
GeneProof MTHFR A1298C PCR Kit M1298/100 real-time PCR 100 reactions

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