GeneProof MTHFR A1298C PCR Kit
CE IVD
PRODUCT DETAIL
The PCR kit is designed to detect A1298C polymorpism in the gene for methylenetetrahydrofolate reductase (MTHFR) by the real-time Polymerase Chain Reaction (PCR).
The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide adenine/cytosine polymorphism in site 1298 (A1298C). Presence of wild-type allele (A1298A) is detected in the FAM fluorescent channel and mutant allele (C1298C) in the HEX fluorescent channel. In case of heterozygous genotype (A1298C) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.
TECHNICAL SPECIFICATION
| Technology | real-time PCR |
| Type of Analysis | qualitative |
| Target Sequence | A1298C mutation in the gene for methylenetetrahydrofolate reductase (MTHFR) |
| Analytical Sensitivity | reaches up to 2 ng/µl |
| Diagnostic Specificity | 100 % |
| Diagnostic Sensitivity | 100 % |
| Validated Specimen | whole blood |
| Storage | -20 ± 5 °C |
| Validated Extraction Methods | croBEE NA16 Nucleic Acid Extraction System GeneProof PathogenFree DNA Isolation Kit |
| Validated Instruments | croBEE Real-Time PCR System Applied Biosystems 7300 / 7500 Real-Time PCR System AriaMx Real-Time PCR System CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System LightCycler® 2.0 LineGene 9600 / 9600 Plus |
Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / Q
SLAN® Real-Time PCR SystemRequired Detection ChannelsFAM, HEXExternal Quality AssessmentRegularly tested by Instand e.V. External Quality Assessment panels – results at www.geneproof.comCertificationCE IVD for in vitro Diagnostic Use
ORDER INFORMATION
| Product name | Cat. No. | Technology | Package |
| GeneProof MTHFR A1298C PCR Kit | M1298/050 | real-time PCR | 50 reactions |
| GeneProof MTHFR A1298C PCR Kit | M1298/100 | real-time PCR | 100 reactions |