GeneProof MTHFR C677T PCR Kit

GeneProof MTHFR C677T PCR Kit


thrombotic mutations


The PCR kit is designed to detect C677T polymorphism in the gene for methylenetetrahydrofolate reductase (MTHFR) by the real-time Polymeras Chain Reaction (PCR).

The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide cytosine/thymine polymorphism in site 677 (C677T). Presence of wild-type allele (C677C) is detected in the FAM fluorescent channel and mutant allele (T677T) in the HEX fluorescent channel. In case of heterozygous genotype (C677T) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.



Technology real-time PCR
Type of Analysis qualitative
Target Sequence C677T mutation in the gene for methylenetetrahydrofolate reductase (MTHFR)
Analytical Sensitivity reaches up to 2 ng/µl
Diagnostic Specificity 100 %
Diagnostic Sensitivity 100 %
Validated Specimen whole blood
Storage -20 ± 5 °C
Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
Instruments croBEE Real-Time PCR System*
Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
LightCycler® 2.0 / 480
LineGene 9600 PlusMic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / 6000
SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR System
Required Detection Channels FAM, HEX
External Quality Assessment regularly tested by Instand e.V. External Quality Assessment panels – results at
Certification CE IVD for in vitro Diagnostic Use

* Validated Instruments


Product name Cat. No. Technology Package
GeneProof MTHFR C677T PCR Kit M677/050 real-time PCR 50 reactions
GeneProof MTHFR C677T PCR Kit M677/100 real-time PCR 100 reactions

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