GeneProof Mycoplasma genitalium/hominis PCR Kit

    CE IVD

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The PCR kit is designed for Mycoplasma genitalium and Mycoplasma hominis detection and differentiation by the real-time Polymerase Chain Reaction method (PCR).

The M. genitalium and M. hominis detection and differentiation consist in amplification of a multicopy sequence of the gene encoding the 16S rRNA (M. genitalium) and the housekeeping gap gene for glyceraldehyde-3-phosphate dehydrogenase (M. hominis) and in measurement of fluorescence increase. The M. genitalium presence is indicated by the FAM fluorophore fluorescence growth and the M. hominis presence is indicated by the Cy5 fluorophore fluorescence growth. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR by amplification products. The kit is designed for in vitro diagnostics and provides qualitative detection.
 

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