GeneProof PAI-1 Genotyping PCR Kit

GeneProof PAI-1 Genotyping PCR Kit

    CE IVD 

thrombotic mutations


PRODUCT DETAIL                                            

The PCR kit is designed to detect polymorphism in promoter of the PAI-1 gene by the real-time Polymerase Chain Reaction (PCR) method.

The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is insertion-deletion polymorphism of guanosine 4G/5G in site 675. Presence of wild-type allele (5G/5G) is detected in the FAM fluorescent channel and mutant allele (4G/4G) in the HEX fluorescent channel. In case of heterozygous genotype (4G/5G) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostic.


Technology real-time PCR
Type of Analysis qualitative
Target Sequence polymorphism in promoter of the PAI-1 gene in site 675
Analytical Sensitivity reaches up to 2 ng/µl
Diagnostic Specificity 100 %
Diagnostic Sensitivity 100 %
Validated Specimen whole blood
Storage -20 ± 5 °C
Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
Instruments croBEE Real-Time PCR System*
Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System
LightCycler® 2.0 / 480
LineGene 9600 Plus

Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / Q
SLAN® Real-Time PCR SystemRequired Detection ChannelsFAM, HEXExternal Quality AssessmentRegularly tested by Instand e.V. External Quality Assessment Panels - results at www.geneproof.comCertificationCE IVD for in vitro Diagnostic Use

* Validated Instruments


Product name Cat. No. Technology Package
GeneProof PAI-1 Genotyping PCR Kit PAI/050 real-time PCR 50 reactions
GeneProof PAI-1 Genotyping PCR Kit PAI/100 real-time PCR 100 reactions

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