GeneProof PAI-1 Genotyping PCR Kit
CE IVD
PRODUCT DETAIL
The PCR kit is designed to detect polymorphism in promoter of the PAI-1 gene by the real-time Polymerase Chain Reaction (PCR) method.
The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is insertion-deletion polymorphism of guanosine 4G/5G in site 675. Presence of wild-type allele (5G/5G) is detected in the FAM fluorescent channel and mutant allele (4G/4G) in the HEX fluorescent channel. In case of heterozygous genotype (4G/5G) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostic.
TECHNICAL SPECIFICATION
| Technology | real-time PCR |
| Type of Analysis | qualitative |
| Target Sequence | polymorphism in promoter of the PAI-1 gene in site 675 |
| Analytical Sensitivity | reaches up to 2 ng/µl |
| Diagnostic Specificity | 100 % |
| Diagnostic Sensitivity | 100 % |
| Validated Specimen | whole blood |
| Storage | -20 ± 5 °C |
| Validated Extraction Methods | croBEE NA16 Nucleic Acid Extraction System GeneProof PathogenFree DNA Isolation Kit |
| Instruments | croBEE Real-Time PCR System* Applied Biosystems 7300 / 7500 Real-Time PCR System AriaMx Real-Time PCR System CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System LightCycler® 2.0 / 480 LineGene 9600 Plus |
Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / Q
SLAN® Real-Time PCR SystemRequired Detection ChannelsFAM, HEXExternal Quality AssessmentRegularly tested by Instand e.V. External Quality Assessment Panels - results at www.geneproof.comCertificationCE IVD for in vitro Diagnostic Use
* Validated Instruments
ORDER INFORMATION
| Product name | Cat. No. | Technology | Package |
| GeneProof PAI-1 Genotyping PCR Kit | PAI/050 | real-time PCR | 50 reactions |
| GeneProof PAI-1 Genotyping PCR Kit | PAI/100 | real-time PCR | 100 reactions |