GeneProof VRE PCR Kit

CE IVD

PRODUCT DETAIL

The PCR kit is designed for the detection of Vancomycin-Resistant Enterococci (VRE), e. g. Enterococcus faecalis and Enterococcus faecium, by the real-time Polymerase Chain Reaction (PCR) method.

The VRE detection consists in amplification of a specific conservative DNA sequence in the area of the vanA and vanB genes and in measurement of fluorescence increase. The vanA gene presence is indicated by the FAM fluorophore fluorescence growth and the vanB gene presence is indicated by the Cy5 fluorophore fluorescence growth. An Internal Standard (IS)          is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR with amplification products. The kit is designed for in vitro diagnostics and provides qualitative detection.

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