GeneProof Ureaplasma PCR Kit

CE IVD

PRODUCT DETAIL

The PCR kit is designed for Ureaplasma parvum and Ureaplasma urealyticum detection and differentiation by the real-time Polymerase Chain Reaction (PCR) method.

The U. parvum and U. urealyticum detection and differentiation consist in amplification of a conservative sequence of UreD gene and in measurement of fluorescence increase. The U. parvum presence is indicated by the FAM fluorophore fluorescence growth only. The U. urealyticum presence is indicated by the fluorescence growth in both FAM and Cy5 channels. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA glycosylase (UDG), eliminating possible contamination of the PCR with amplification products. The kit is designed for in vitro diagnostics and provides qualitative detection.
 

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